categorised in CHEMICAL , MECHANICAL, OTHER OR BOTH

(legend needed) allows to

Relevant Illustrations and theory are cleverly inserted for the user's information

fferent structural modifications enable G-proteins to do what they do

GPCRs to G-proteins involves specific regions of the recepto

introduces the ligand to the appropriate binding site within the GPCR structure

N-terminal tip is cleaved by an enzyme (serum serine proteinase),

sequestration and modulation of their local concentratio

binds to one N-terminal peptide

second N-terminal peptide binds to the second monomer leading to the formation of a symmetric 2:2 complex

Negative allosteric modulators

increase or decrease receptor activity  (pos

ight look like a competitive irreversible antagonist.

gonists, k-1/k+1 can be expressed as the equilibrium/dissociation constant.

oth have affinity for receptors!

So you can use the same equation to describe competitive (reversible) antagonist binding to a recepto

allergen binds to sensitized mast cells (and basophils) it causes a massive degranulation

interesting points will all be squashed in close to the Y-axis

computing the EC50 (th

this pathway activates PLC which increases production of the second messengers IP3 and DAG, eventually leading to the release of Ca2+ from intracellular stores

n can neatly explain the interaction of PE with the α1-adrenoceptor (we'll cover this in excruciating detail in the lectures), b

measure mRNA levels.

main advantages, and limitation, of measuring mRNA levels